Journal: Frontiers in Microbiology

Article Title: Genome-wide transcription response of Staphylococcus epidermidis to heat shock and medically relevant glucose levels

doi: 10.3389/fmicb.2024.1408796

Figure Lengend Snippet: Environmental perturbation of S. epidermidis . Log-phase cultures were exposed to (A) a 10-min increase in temperature from 37 to 45°C or (B) a range of 20-min glucose spikes (concentrations as noted) and a 10 mM spike followed by a step down to 2 mM.

Article Snippet: As a second example, Azitra, Inc. indicates they are engineering S. epidermidis strains to deliver therapeutic proteins to treat skin diseases including Netherton Syndrome and to improve skin appearance ( ).

Techniques:

Journal: Frontiers in Microbiology

Article Title: Genome-wide transcription response of Staphylococcus epidermidis to heat shock and medically relevant glucose levels

doi: 10.3389/fmicb.2024.1408796

Figure Lengend Snippet: A sudden temperature increase causes transcript levels to change up to ~ 71-fold. (A) Volcano plot showing the differentially expressed genes (DEGs) for the heat-shock experimental condition with |log2 FC| ≥ 1.5 and adjusted p ≤ 0.05 as the threshold. The red dots represent 235 significantly upregulated genes, and the blue dots represent 131 significantly downregulated genes. (B) Summary of the significantly upregulated and downregulated genes during the heat-shock response in S. epidermidis assigned to functional groups according to GO and KEGG pathways (in %).

Article Snippet: As a second example, Azitra, Inc. indicates they are engineering S. epidermidis strains to deliver therapeutic proteins to treat skin diseases including Netherton Syndrome and to improve skin appearance ( ).

Techniques: Functional Assay

Journal: Frontiers in Microbiology

Article Title: Genome-wide transcription response of Staphylococcus epidermidis to heat shock and medically relevant glucose levels

doi: 10.3389/fmicb.2024.1408796

Figure Lengend Snippet: Eighty-five S. epidermidis genes change expression levels in response to glucose. (A) Circular transcriptome map showing normalized gene expression levels in the S. epidermidis genome in response to glucose. Log2 fold change relative to control for cells exposed to 2 mM (G2), 5 mM (G5), 10 mM (G10), 17 mM (G17), or 50 mM (G50) glucose spikes. Each bar denotes a single gene; red bars represent significantly upregulated genes and blue bars represent significantly downregulated genes. Roman numerals i ( sdaAB , rbsU ), ii ( pflB ), iii ( glpR-pfkB operon), iv (F1613_RS07845 (homoserine dehydrogenase), and v (members of the lac operon) correspond to select groups of genes that are downregulated across all five glucose spike conditions. (B) Glucose concentration-response curves for a representative subset of genes that have potentially interesting glucose-responsive switch properties.

Article Snippet: As a second example, Azitra, Inc. indicates they are engineering S. epidermidis strains to deliver therapeutic proteins to treat skin diseases including Netherton Syndrome and to improve skin appearance ( ).

Techniques: Expressing, Gene Expression, Control, Concentration Assay

Journal: Frontiers in Microbiology

Article Title: Genome-wide transcription response of Staphylococcus epidermidis to heat shock and medically relevant glucose levels

doi: 10.3389/fmicb.2024.1408796

Figure Lengend Snippet: The genome-wide transcription response of S. epidermidis to perturbations. Circular transcriptome map showing normalized gene expression levels in the S. epidermidis genome. Log2-fold change relative to control for cells exposed to Heat Shock (HS), a 17 mM glucose spike (G17), or Step Down (SD) experimental conditions. Each bar denotes a single gene. The red bars represent significantly upregulated genes, and the blue bars represent significantly downregulated genes.

Article Snippet: As a second example, Azitra, Inc. indicates they are engineering S. epidermidis strains to deliver therapeutic proteins to treat skin diseases including Netherton Syndrome and to improve skin appearance ( ).

Techniques: Genome Wide, Gene Expression, Control

Journal: Cell reports

Article Title: Staphylococcus epidermidis activates keratinocyte cytokine expression and promotes skin inflammation through the production of phenol-soluble modulins

doi: 10.1016/j.celrep.2023.113024

Figure Lengend Snippet:

Article Snippet: S. epidermidis PSMα, fMADVIAKIVEIVKGLIDQFTQK , Lifetein , N/A.

Techniques: Staining, Virus, Recombinant, SYBR Green Assay, cDNA Synthesis, DNA Purification, Enzyme-linked Immunosorbent Assay, Plasmid Preparation, Software